鉴定与水稻光敏核不育基因pms3连锁的AFLP-RFLP标记

陈亮, 梅明华, 徐才国, 王伟, 崔红
厦门大学学报(自然科学版), 2000, 39(4): 421-425     追溯原文......本站官方QQ群:62473826
AFLP-RFLP; pms3; 水稻

运用AFLP技术对农垦58S×1514的F2 代群体构建的极端集团进行了分析。在253对AFLP引物中,91%具有多态性带,有20对引物扩增到了阳性带。阳性带经克隆后用作RFLP探针进行极端集团分析,其中4个具有多态性带,2个(F3和V4)表现为阳性带。F2 代不育群体RFLP分析结果显示,F3和V4两个标记与第12染色体上的光敏核不育基因pms3 连锁。通过极大似然法估算,F3和V4标记距光敏核不育基因pms3 的遗传距离分别为5.80cM 和7.75cM。

Identification of AFLP-RFLP Makers Linked to the Photoperiod-sensitive Male Sterile Gene pms3 in Rice

CNEN Liang, WEI Ming-hua, XU Cai-guo, WANG Wei, CUI Hong
Journal of Xiamen University(Natural Science), 2000, 39(4): 421-425
AFLP-RFLP; pms3; rice

The bulked DNA from the extreme fertile and sterile plants of the F2 population and the parents DNA as NongKen58S×1514 were analyzed via AFLP technique. The polymorphic bands amplified were 91% with 253 AFLP primer pairs, among which 20 AFLP primer pairs gave positive bands. These positive bands were later cloned and transformed into RFLP probes used in the bulked extremes analysis. Among these 20 RFLP markers there were 4 appearing polymorphic bands and 2 positive makers, F3 and V4. F3 and V4 were linked to the PGMS gene located pms3 in Chromosome 12 via RFLP analysis of the F2 sterile population. The genetic distance calculated by Maximum likelihood were 5.80 cM and 7.75 cM to pms3, respectively.


基因列表
  光温敏雄性核不育基因 pms3; p/tms12-1; IncRNA; LDM