水稻光敏核不育基因pms3的精细定位

李香花, 王伏林, 陆青, 徐才国
作物学报, 2002, 28(3): 310-314  DOI: 10.3321/j.issn:0496-3490.2002.03.005;      追溯原文......本站官方QQ群:62473826
光敏核不育水稻; 花药培养; 偏分离; 基因定位; RFLP标记
华中农业大学作物遗传改良国家重点实验室

为了进一步研究水稻晚粳品种农垦58转变为光敏核不育水稻农垦58S的突变位点,并精细定位光敏不育基因pms3,我们利用农垦58S/1514杂交组合的F1进行花药培养构建了一个DH群体,验证了在该群体中光敏不育受pms1pms3 两对基因的控制,并根据分子标记分析选择第12染色体是1514基因型、第7染色体农垦58S基因型的DH系DH80与农垦58S杂交构建一个pms3 光敏不育单基因分离的群体。根据F3家系的育性分离情况推测出F2 的基因型,结合分子标记分析进一步验证了pms3在第12染色体上的位置,并将其定位在RFLP标记M36和RZ261之间,与两标记的遗传距离分别为1.5cM和3.05cM,为启动pms3区域的染色体步查打下了基础。同时还比较分析了RFLP标记在DH群体及F2 群体中的分离情况,发现第12染色体上pms3 区域在F2群体中正常分离的多个标记在DH群体中发生了严重的偏分离,另外还发现DH群体第3、7、11染色体上的标记也发生了偏分离。

Fine Mapping of PSGMS Gene pms3 in Rice (Oryza sativa L.)

LI Xiang-Hua, WANG Fu-Lin, LU Qing, XU Cai-Guo
Acta Agronomica Sinica, 2002, 28(3): 310-314
PSGMS ( photo-sensit ive genic male sterile) rice; Anther culture; Marker seg regated distort ion; Gene locat ion; RFLPs

In order to determine the precise location of pms3 gene which induce Nongken58 (NK58) to PSGMS Nongken58S (NK58S) , a DH population was constructed by culturing F1 anthers of NK58S/ 1514. A line DH80, which was only with PSGMS gene pms1, was selected to cross with NK58S for constructing a segregation population only in pms3 locus. Segregation of RFLP markers was compared between DH and F2 populations. And the results showed that several markers close to pms3 on chromosome 12 normally segregated in F2 populat ion showed obviously distortion segregation in DH population. Meanwhile, some markers on chromosome 3, 7 and 11 in DH population also segregated distortedly. The genotypes for every F3 family were determined by analysis of fertility segregation. And then the location of pms3 and distances between RFLP markers and pms3 were precisely calculated. The gene pms3 is located between M36 and RZ261, but much closer to M36.


基因列表
  光温敏雄性核不育基因 pms3; p/tms12-1; IncRNA; LDM