Photoperiod- and thermo-sensitive genic male sterility in rice are caused by a point mutation in a novel noncoding RNA that produces a small RNA

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Hai Zhou, Qinjian Liu, Jing Li, Dagang Jiang, Lingyan Zhou, Ping Wu, Sen Lu, Feng Li, Liya Zhu, Zhenlan Liu, Letian Chen, Yao-Guang Liu, Chuxiong Zhuang
Cell Research, 2012, 22(4): 649-660  DOI: 10.1038/cr.2012.28;      追溯原文......本站官方QQ群:62473826
rice; PGMS; TGMS; noncoding RNA; small RNA

Photoperiod- and thermo-sensitive genic male sterility (PGMS and TGMS) are the core components for hybrid breeding in crops. Hybrid rice based on the two-line system using PGMS and TGMS lines has been successfully developed and applied widely in agriculture. However, the molecular mechanism underlying the control of PGMS and TGMS remains obscure. In this study, we mapped and cloned a major locus, p/tms12-1 (photo- or thermo-sensitive genic male sterility locus on chromosome 12), which confers PGMS in the japonica rice line Nongken 58S (NK58S) and TGMS in the indica rice line Peiai 64S (PA64S, derived from NK58S). A 2.4-kb DNA fragment containing the wild-type allele P/TMS12-1 was able to restore the pollen fertility of NK58S and PA64S plants in genetic complementation. P/TMS12-1 encodes a unique noncoding RNA, which produces a 21-nucleotide small RNA that we named osa-smR5864w. A substitution of C-to-G in p/tms12-1, the only polymorphism relative to P/TMS12-1, is present in the mutant small RNA, namely osa-smR5864m. Furthermore, overexpression of a 375-bp sequence of P/TMS12-1 in transgenic NK58S and PA64S plants also produced osa-smR5864w and restored pollen fertility. The small RNA was expressed preferentially in young panicles, but its expression was not markedly affected by different day lengths or temperatures. Our results reveal that the point mutation in p/tms12-1, which probably leads to a loss-of-function for osa-smR5864m, constitutes a common cause for PGMS and TGMS in the japonica and indica lines, respectively. Our findings thus suggest that this noncoding small RNA gene is an important regulator of male development controlled by cross-talk between the genetic networks and environmental conditions.

水稻光温敏遗传雄性不育是由一个新的产生小RNA的非编码RNA中一个点突变引起的

光周期和温度敏感遗传雄性不育(PGMS和TGMS)是作物杂交育种的核心元件。基于PGMS和TGMS的两系杂交水稻已经成功地发展和运用于农业生产。但是对于PGMS和TGMS调控的分子遗传机理还不清楚。本研究我们对引起粳稻品系农垦58S(NK58S)PGMS和籼稻品系培矮64S(PA64S,由NK58S衍生而来)TGMS的主效座位p/tms12-1进行了定位和克隆。互补实验表明包含野生型P/TMS12-1等位基因的一个2.4kb的DNA片段能够恢复NK58S和PA64S的花粉育性。P/TMS12-1编码一个特异的非编码RNA,产生21nt的小RNA,我们将之命名为osa-smR5864w。和P/TMS12-1相比,p/tms12-1中有一个C-G替换出现在突变型小RNA内,我们将之命名为osa-smR5864m。此外,在NK58S和PA64S中过量表达一个375bp可以产生osa-smR5864w的P/TMS12-1序列也能回复花粉育性。这个小RNA主要在幼穗表达,但是其表达不受日长或温度的显著影响。我们的结果揭示了p/tms12-1中一个点突变导致了osa-smR5864m的功能丧失,这是分别引起粳稻和籼稻品系PGMS和TGMS的共同原因。因此我们的发现表明这个非编码小RNA基因通过遗传网络和环境调节间的串话,是控制雄配子发育的一个重要调节因子。


基因列表
  光温敏雄性核不育基因 pms3; p/tms12-1; IncRNA; LDM