A long noncoding RNA regulates photoperiod-sensitive male sterility, an essential component of hybrid rice

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Jihua Ding, Qing Lu, Yidan Ouyang, Hailiang Mao, Pingbo Zhang, Jialing Yao, Caiguo Xu, Xianghua Li, Jinghua Xiao, Qifa Zhang
Proceedings of the National Academy of Sciences, 2012, 109(7): 2654-2659  DOI: 10.1073/pnas.1121374109;      追溯原文......本站官方QQ群:62473826

Hybrid rice has greatly contributed to the global increase of rice productivity. A major component that facilitated the development of hybrids was a mutant showing photoperiod-sensitive male sterility (PSMS) with its fertility regulated by day length. Transcriptome studies have shown that large portions of the eukaryotic genomic sequences are transcribed to long noncoding RNAs (lncRNAs). However, the potential roles for only a few lncRNAs have been brought to light at present. Thus, great efforts have to be invested to understand the biological functions of lncRNAs. Here we show that a lncRNA of 1,236 bases in length, referred to as long-day–specific male-fertility–associated RNA (LDMAR), regulates PSMS in rice. We found that sufficient amount of the LDMAR transcript is required for normal pollen development of plants grown under long-day conditions. A spontaneous mutation causing a single nucleotide polymorphism (SNP) between the wild-type and mutant altered the secondary structure of LDMAR. This change brought about increased methylation in the putative promoter region of LDMAR, which reduced the transcription of LDMAR specifically under long-day conditions, resulting in premature programmed cell death (PCD) in developing anthers, thus causing PSMS. Thus, a lncRNA could directly exert a major effect on a trait like a structure gene, and a SNP could alter the function of a lncRNA similar to amino acid substitution in structural genes. Molecular elucidating of PSMS has important implications for understanding molecular mechanisms of photoperiod regulation of many biological processes and also for developing male sterile germplasms for hybrid crop breeding.

一个长非编码RNA调控光敏感雄性不育,是杂交水稻的必需组分

杂交水稻极大地推动水稻粮食产量的提高。其中一个可受日照长度调控的光敏雄性不育PSMS突变体的应用对杂交水稻的发展起到关键的作用。转录组分析发现真核基因组的序列的很大部分是转录成长链的非编码RNAs(IncRNAs)。然而,当前我们仅发现了一些IncRNA的潜在作用,还需要努力去了解探索更多的IncRNAs生物学功能。本文中,我们发现了一个长度为1236bp,且与长光照下特异的雄性不育相关的RNA分子(LDMAR),其调控这水稻光敏雄性不育PSMS表型。长日照条件下,足够的LDMAR转录量是维持正常花粉发育所必需的。但由于一个自发的单碱基突变造成LDMAR的二级结构的改变,导致了LDMAR在特异的长日照下转录量降低,结果造成正处于发育的花药进入程序化死亡(PCD)提前即产生PSMS。该IncRNA类似一个结构基因直接控制该表现,该SNP如同结构基因氨基酸的替换一样也能导致了IncRNA功能的变化。该PSMS的基因发现让我们对许多光敏调控的分子机制有了更深入的了解,也有利于雄性不育的种资资源在杂交水稻育种的利用。


基因列表
  光温敏雄性核不育基因 pms3; p/tms12-1; IncRNA; LDM