Fine mapping and candidate gene prediction of the photoperiod and thermo-sensitive genic male sterile gene pms1(t) in rice

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Yuan-fei Zhou, Xian-yin Zhang, Qing-zhong Xue
Journal of Zhejiang University SCIENCE B, 2011, 12(6): 436-447  DOI: 10.1631/jzus.B1000306;      追溯原文......本站官方QQ群:62473826
Super hybrid rice – pms1(t) – Photoperiod and thermo-sensitive male sterile gene – Genetic mapping

Pei'ai64S, an indica sterile variety with photoperiod and thermo-sensitive genic male sterile (PTGMS) genes, has been widely exploited for commercial seed production for “two-line” hybrid rice in China. One PTGMS gene from Pei'ai64S, pms1(t), was mapped by a strategy of bulked-extreme and recessive-class approach with simple sequence repeat (SSR) and insert and deletion (In-Del) markers. Using linkage analysis for the F2 mapping population consisting of 320 completely male sterile individuals derived from a cross between Pei'ai64S and 93-11 (indica restorer) lines, the pms1(t) gene was delimited to the region between the RM21242 (0.2 cM) and YF11 (0.2 cM) markers on the short arm of chromosome 7. The interval containing the pms1(t) locus, which was co-segregated with RM6776, is a 101.1 kb region based on the Nipponbare rice genome. Fourteen predicted loci were found in this region by the Institute for Genomic Research (TIGR) Genomic Annotation. Based on the function of the locus LOC_Os07g12130 by bioinformatics analysis, it is predicted to encode a protein containing a Myb-like DNA-binding domain, and may process the transcript with thermosensory response. The reverse transcription-polymerase chain reaction (RT-PCR) results revealed that the mRNA levels of LOC_Os07g12130 were altered in different photoperiod and temperature treatments. Thus, the LOC_Os07g12130 locus is the most likely candidate gene for pms1(t). These results may facilitate not only using the molecular marker assisted selection of PTGMS genes, but also cloning of the pms1(t) gene itself.

水稻光温敏雄性核不育基因pms1(t)的精细定位及候选基因预测

培矮64S是一个带有光温敏雄性核不育(PTGMS)基因的籼型不育系,在中国两系杂交水稻制种中被广泛应用。应用隐性极端群体法,结合简单序列重复(SSR)标记和插入缺失(In-Del)标记,定位了一个控制培矮64S育性的PTGMS基因,pms1(t)。利用来源于培矮64S和9311(籼型恢复系)杂交的含有320个完全不育单株的F2定位群体,通过连锁分析,将pms1(t)定位在水稻第7染色体短臂标记RM21242 (0.2 cM) 和YF11 (0.2 cM)之间。基于日本晴基因组,该区间为101.1 kb,包含pms1(t)基因位点,目的基因与RM6776共分离。基于TIGR基因组注释,该区间内包含14个基因。基于生物信息学对LOC_Os07g12130位点进行功能分析,其编码一个含有1个Myb-like DNA-binding结构域的蛋白,并可能参与热敏感响应的转录过程。RT-PCR结果显示,LOC_Os07g12130在不同光周期和温度处理下,其转录水平不同。所以,LOC_Os07g12130被认为是pms1(t)的最有可能的候选基因。本研究将为分子标记辅助选择利用PTGMS基因,及pms1(t)的克隆奠定基础。


基因列表
  光敏雄性核不育基因 pms1