Inducible overexpression of Ideal Plant Architecture1 improves both yield and disease resistance in rice

Mingming Liu, Zhenying Shi, Xiaohan Zhang, Mingxuan Wang, Lin Zhang, Kezhi Zheng, Jiyun Liu, Xingming Hu, Cuiru Di, Qian Qian, Zuhua He, Dong-Lei Yang
Nature Plants, 2019, 5 : 389-400  DOI: 10.1038/s41477-019-0383-2;      追溯原文......本站官方QQ群:62473826

Breeding crops with resistance is an efficient way to control diseases. However, increased resistance often has a fitness penalty. Thus, simultaneously increasing disease resistance and yield potential is a challenge in crop breeding. In this study, we found that downregulation of microRNA-156 (miR-156) and overexpression of Ideal Plant Architecture1 (IPA1) and OsSPL7, two target genes of miR-156, enhanced disease resistance against bacterial blight caused by Xanthomonas oryzae pv. oryzae (Xoo), but reduced rice yield. We discovered that gibberellin signalling might be partially responsible for the disease resistance and developmental defects in IPA1 overexpressors. We then generated transgenic rice plants expressing IPA1 with the pathogen-inducible promoter of OsHEN1; these plants had both enhanced disease resistance and enhanced yield-related traits. Thus, we have identified miR-156–IPA1 as a novel regulator of the crosstalk between growth and defence, and we have established a new strategy for obtaining both high disease resistance and high yield.

诱导理想株型基因IPA1过度表达提高了水稻产量和抗病性

培育抗病作物是防治疾病的有效途径。然而,抗性的增强往往会导致产量下降。因此,同时提高抗病性和产量潜力对于作物育种来说是一种挑战。通过下调miR156和过表达miR156的两个靶基因IPA1OsSPL7,大幅增强了水稻对白叶枯病的抗性,但造成水稻产量下降。我们发现赤霉素信号可能部分地与IPA1过表达株系的抗病性和发育缺陷有关。然后,我们用OsHEN1的病菌诱导性启动子产生表达IPA1的转基因水稻植株,这些植株的抗病性增强,产量相关性状增加。因此,我们将miR-156–IPA1确定为生长和防卫之间的新调节因子,并建立了获得高抗病性和高产量的新策略。


基因列表
  Squamosa启动子结合蛋白基因; 理想株型基因 OsSPL14; IPA1;WFP