Enhanced expression of OsSPL14 gene and its association with yield components in rice (Oryza sativa) under low nitrogen conditions

B.Srikanth, I.Subhakara Rao, K.Surekha, D.Subrahmanyam, S.R.Voleti, C.N.Neeraja
Gene, 2016, 576(1): 441-450  DOI: 10.1016/j.gene.2015.10.062;      追溯原文......本站官方QQ群:62473826
Rice; Low nitrogen; Yield components; OsSPL14 expression; NUE

Nitrogen use efficiency (NUE) in rice crop is the need of the hour for reduction of nitrous oxide emission resulting from excess nitrogen (N) fertilizer application and also in reduction of cost of cultivation. Ten rice genotypes were grown under low and recommended dose of N application and characterized in terms of parameters related to yield, yield related components and NUE indicators. Wide genetic variability under low N conditions was observed with significant variation for 15 yield related parameters in interactions of genotypes and treatment. Limitation of N has led to the decrease of all yield and yield related parameters, but for grain filling % and 1000 grain weight. Two genotypes, Rasi and Varadhan have shown minimum differences between low and recommended N conditions. Correlation analysis of various yield components showed the importance of the secondary branches for the total grains under low N. Expression analysis of OsSPL14 (LOC_Os08g39890) gene reported to be associated with increased panicle branching and higher grain yield through real time PCR in leaf and three stages of panicle has shown differential temporal expression and its association with yield and yield related components across the genotypes. The expression of OsSPL14 at panicle stage 3, has shown correlation (P < 0.05) with N% in grain. Since OsSPL14 is a functional transcription activator, its association of expression in leaf and three panicle stages with yield components as observed in the present study suggests the role of nitrogen metabolism related genes in plant growth and development and its conversion into yield components in rice.

低氮条件下水稻中osspl14基因的增强表达及其与产量组分的关系

水稻氮素利用效率(NUE)是减少过量施氮肥产生的氧化亚氮排放的需要,也是降低种植成本的需要。10个水稻基因型在低剂量和推荐施氮量下生长,并在产量相关参数、产量相关成分和NUE指标方面进行了描述。


基因列表
  Squamosa启动子结合蛋白基因; 理想株型基因 OsSPL14; IPA1;WFP