A natural tandem array alleviates epigenetic repression of IPA1 and leads to superior yielding rice

Lin Zhang, Hong Yu, Bin Ma, Guifu Liu, Jianjun Wang, Junmin Wang, Rongcun Gao, Jinjun Li, Jiyun Liu, Jing Xu, Yingying Zhang, Qun Li, Xuehui Huang, Jianlong Xu, Jianming Li, Qian Qian, Bin Han, Zuhua He, Jiayang Li
Nature Communications, 2017, 8 : 14789  DOI: 10.1038/ncomms14789     追溯原文......本站官方QQ群:62473826

Super hybrid rice varieties with ideal plant architecture (IPA) have been critical in enhancing food security worldwide. However, the molecular mechanisms underlying their improved yield remain unclear. Here, we report the identification of a QTL, qWS8/ipa1-2D, in the super rice Yongyou12 (YY12) and related varieties. In-depth genetic molecular characterization of qWS8/ipa1-2D reveals that this newly identified QTL results from three distal naturally occurring tandem repeats upstream of IPA1, a key gene/locus previously shown to shape rice ideal plant architecture and greatly enhance grain yield. The qWS8/ipa1-2D locus is associated with reduced DNA methylation and a more open chromatin state at the IPA1 promoter, thus alleviating the epigenetic repression of IPA1 mediated by nearby heterochromatin. Our findings reveal that IPA traits can be fine-tuned by manipulating IPA1 expression and that an optimal IPA1 expression/dose may lead to an ideal yield, demonstrating a practical approach to efficiently design elite super rice varieties.

一个自然的串联序列减轻对IPA1的表观遗传抑制,进而产生超高产水稻

具有理想株型的超级杂交稻品种对于增加全球粮食安全至关重要。但是,它们产量提高的潜在分子机制仍不清楚。本文,我们报道对超级稻甬优12和相关品种中一个QTL的鉴定,命名为qWS8/ipa1-2D。对qWS8/ipa1-2D深入的遗传分子分析表明,这个新鉴定的QTL来源于IPA1上游末端的3个自然发生的串联重复序列,IPA1是一个之前鉴定关键基因/位点,影响水稻理想株型形成并极大增强谷物产量。qWS8/ipa1-2D位点与DNA甲基化减弱相关,IPA1启动子具有更开放的染色体状态,因而通过附近的异染色质减轻对IPA1的表观遗传抑制。我们的结果揭示了通过调控IPA1的表达控制IPA性状,最佳的IPA1表达/剂量能够产生高产水稻,证明了有效设计超级稻品种的实际方法。


基因列表
  Squamosa启动子结合蛋白基因 OsSPL14; IPA1;WFP