Development and validation of co-dominant gene based markers for Pi9, a gene governing broad-spectrum resistance against blast disease in rice

R. Rathour, S. Gopala Krishnan, Apurva Khanna, Swaranjali Dhatwalia, A. Kaachra, T. R. Sharma, Ashok K. Singh
Molecular Breeding, 2016, 36 : 168  DOI: 10.1007/s11032-016-0599-z     追溯原文......本站官方QQ群:62473826
Cleaved amplified polymorphic sequences (CAPS) marker; Gene-based marker; Co-dominant; Marker assisted selection; Rice blast

Rice production and grain quality are severely affected by blast disease caused by the ascomycetous fungus Magnaporthe oryzae. Incorporation of genes that confer broad-spectrum resistance to blast has been a priority area in rice breeding programs. The blast resistance gene Pi9 sourced from Oryza minuta has shown broad spectrum and durable resistance to blast world-wide. In the present study co-dominant gene-based markers were developed for the precise marker-assisted tracking of Pi9 in breeding programs. The developed markers were validated across a diverse set of cultivars including basmati, indica and japonica varieties. Two markers, Pi9STS-1 and Pi9STS-2, effectively differentiated Pi9 donors from all the indicas and commercial basmati varieties tested. However, these markers were monomorphic between Pi-9 donors (IRBL9-W and Pusa 1637) and japonica type varieties. An additional gene-derived CAPS marker Pi91F_ 2R was developed to differentiate Pi9 donors from japonicas and traditional basmati lines. The co-dominant markers developed in the present study will be of immense utility to rice breeders for precise and speedy incorporation of Pi-9 into susceptible rice varieties through marker-assisted selection.

  稻瘟病抗性基因 Pi9; Pi2/Piz-5; Pi50; Piz; Pi