Positional Cloning of Rice Semidwarfing Gene, sd-1: Rice "Green Revolution Gene" Encodes a Mutant Enzyme Involved in Gibberellin Synthesis

Lisa Monna,  Noriyuki Kitazawa,  Rika Yoshino,  Junko Suzuki,  Haruka Masuda,  Yumiko Maehara,  Masao Tanji,  Mizuho Sato,  Shinobu Nasu and Yuzo Minobe
DNA Research, 2002, 9(1): 11-17     追溯原文......本站官方QQ群:62473826
rice (Oryza sativa L.);  positional cloning;  sd-1;  cleaved amplified polymorphic sequence (CAPS);  single nucleotide polymorphism (SNP)

A rice semidwarfing gene, sd-1, known as the "green revolution gene," was isolated by positional cloning and revealed to encode gibberellin 20-oxidase, the key enzyme in the gibberellin biosynthesis pathway. Analysis of 3477 segregants using several PCR-based marker technologies, including cleaved amplified polymorphic sequence, derived-CAPS, and single nucleotide polymorphisms revealed 1 ORF in a 6-kb candidate interval. Normal-type rice cultivars have an identical sequence in this region, consisting of 3 exons (558, 318, and 291 bp) and 2 introns (105 and 1471 bp). Dee-Geo-Woo-Gen-type sd-1 mutants have a 383-bp deletion from the genome (278-bp deletion from the expressed sequence), from the middle of exon 1 to upstream of exon 2, including a 105-bp intron, resulting in a frame-shift that produces a termination codon after the deletion site. The radiation-induced sd-1 mutant Calrose 76 has a 1-bp substitution in exon 2, causing an amino acid substitution (Leu [CTC] to Phe [TTC]). Expression analysis suggests the existence of at least one more locus of gibberellin 20-oxidase which may prevent severe dwarfism from developing in sd-1 mutants.

  半矮秆基因 sd1; OsGA20ox2; qSD1-2