Linking differential domain functions of the GS3 protein to natural variation of grain size in rice

9.771
Hailiang Mao, Shengyuan Sun, Jialing Yao, Chongrong Wang, Sibin Yu, Caiguo Xu, Xianghua Li, Qifa Zhang
Proceedings of the National Academy of Sciences, 2010, 107(45): 19579-19584  DOI: 10.1073/pnas.1014419107     追溯原文......本站官方QQ群:62473826
grain weight; Oryza sativa L.; protein domain; yield

Grain yield in many cereal crops is largely determined by grain size. Here we report the genetic and molecular characterization of GS3, a major quantitative trait locus for grain size. It functions as a negative regulator of grain size and organ size. The wild-type isoform is composed of four putative domains: a plant-specific organ size regulation (OSR) domain in the N terminus, a transmembrane domain, a tumor necrosis factor receptor/nerve growth factor receptor (TNFR/NGFR) family cysteine-rich domain, and a von Willebrand factor type C (VWFC) in the C terminus. These domains function differentially in grain size regulation. The OSR domain is both necessary and sufficient for functioning as a negative regulator. The wild-type allele corresponds to medium grain. Loss of function of OSR results in long grain. The C-terminal TNFR/NGFR and VWFC domains show an inhibitory effect on the OSR function; loss-of-function mutations of these domains produced very short grain. This study linked the functional domains of the GS3 protein to natural variation of grain size in rice.

水稻中GS3蛋白质不同结构域的功能与籽粒大小的自然变异的关联

在许多谷类作物的谷物产量在很大程度上取决于籽粒的大小。在这里,我们报道了GS3的遗传和分子鉴定,一个主效的控制籽粒大小的数量性状基因座。它在控制籽粒和器官大小中发挥着负调节子的功能。野生型等位基因包含有四个推测的结构域:N端是一种植物特有的调节器官大小的结构域(OSR),一个跨膜区,肿瘤坏死因子/神经生长因子受体(TNFR / NGFR)家族富半胱氨酸结构域,以及C端的C型血管性血友病因子(VWFC)。这些结构域在调节籽粒大小中发挥不同的功能。OSR结构域作为一个负调节子发挥作用是充分必要的。野生型等位基因对应形成中等长度的籽粒。OSR结构功能的丢失会导致形成长的籽粒。 C端TNFR/NGFR和VWFC结构域显示出对OSR功能的抑制作用;对这些领域的功能失活突变会产生非常短的籽粒。本研究将GS3蛋白质的结构域的功能与水稻种子籽粒大小的自然变异联系了起来。


基因列表
  粒长粒重主效控制基因 GS3