Marker-assisted breeding of Xa4, Xa21 and Xa27 in the restorer lines of hybrid rice for broad-spectrum and enhanced disease resistance to bacterial blight

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Yanchang Luo, Jatinder S. Sangha, Shouhai Wang, Zefu Li, Jianbo Yang, Zhongchao Yin
Molecular Breeding, 2012, 30(4): 1601-1610  DOI: 10.1007/s11032-012-9742-7     追溯原文......本站官方QQ群:62473826
Bacterial blight; Hybrid rice; Disease resistance; Gene pyramiding; Marker-assisted selection; Xanthomonas oryzae pv. oryzae

Hybrid rice based on heterosis can significantly increase rice yield compared to inbred rice. Bacterial blight (BB) of rice caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the most destructive bacterial diseases that affect hybrid rice production. To breed a broad-spectrum and high disease resistance to BB in hybrid rice, we introduced the Xa4, Xa21 and Xa27 genes into the restorer lines of Mianhui 725 or 9311 genetic backgrounds and pyramided the three R genes in the progeny derived from the cross between the two lines. A near-isogenic line of the Xa27 gene in the genetic background of 9311 [9311(Xa27)] and another line with the Xa4 and Xa21 genes in the genetic background of Mianhui 725 (WH421) were firstly developed through marker-assisted selection. A new restorer line carrying Xa4, Xa21 and Xa27, designated as XH2431, was selected from the F8 progeny of the cross between 9311(Xa27) and WH421 through marker-assisted breeding and pedigree selection. XH2431 and II You 2431, the hybrids derived from cytoplasmic male-sterile line II-32A and restorer line XH2431, conferred high resistance to all 23 Xoo strains collected from 10 countries. XH2431 restored the fertility of II-32A to the normal level in the F1 generation. In addition, II You 2431 showed good agronomic traits under greenhouse conditions. The development of XH2431, 9311(Xa27) and WH421 provides a set of restorer lines with broad-spectrum and enhanced resistance to BB for hybrid rice.

通过分子标记辅助育种将Xa4Xa21Xa27导入杂交稻恢复系增强对白叶枯病的广谱抗病能力

因杂种优势,杂交稻的产量比常规稻有显著提高。白叶枯病菌引起的水稻白叶枯病是对杂交稻生产最具破坏性的细菌性病害之一。为了培育具有广谱抗性的高抗白叶枯病杂交稻品种,我们将Xa4Xa21Xa27基因导入绵恢725或9311为背景的恢复系并通过2个株系的杂交后代将3个R基因叠加。首先通过分子标记辅助选择得到了以9311为背景带有Xa27基因的近等基因系9311(Xa27)和另一个以绵恢725为背景带有Xa4Xa21基因的株系(WH421)。进一步利用分子标记辅助育种和系谱选择从9311(Xa27)和WH421的杂交F8后代中构建一个带有Xa4Xa21Xa27的新恢复系(XH2431)。XH2431和Ⅱ优2431(胞质不育系Ⅱ-32A与恢复系XH2431产生的杂交后代)对来自10个国家的23个白叶枯病菌都具有高抗水平。XH2431与Ⅱ-32A的F1代恢复了正常育性。此外Ⅱ优2431在温室里农艺性状也表现较好。通过对XH2431、9311(Xa27)和WH421的培育,我们提供了一套具有广谱白叶枯病抗性的杂交水稻恢复系。


基因列表
  白叶枯病抗性基因 Xa21; Xa-21
  白叶枯病抗性基因 Xa3; Xa4b; Xaw; Xa6; xa9
  白叶枯病抗性基因 Xa27; Xa-min(t)