Regional Expression of the Rice KN1-Type Homeobox Gene Family during Embryo, Shoot, and Flower Development

Naoki Sentoku, Yutaka Sato, Nori Kurata, Yukihiro Ito, Hidemi Kitano, Makoto Matsuoka
The Plant Cell, 1999, 11(9): 1651-1664     追溯原文......本站官方QQ群:62473826

We report the isolation, sequence, and pattern of gene expression of members of the KNOTTED1 (KN1)-type class 1 homeobox gene family from rice. Phylogenetic analysis and mapping of the rice genome revealed that all of the rice homeobox genes that we have isolated have one or two direct homologs in maize. Of the homeobox genes that we tested, all exhibited expression in a restricted region of the embryo that defines the position at which the shoot apical meristem (SAM) would eventually develop, prior to visible organ formation. Several distinct spatial and temporal expression patterns were observed for the different genes in this region. After shoot formation, the expression patterns of these homeobox genes were variable in the region of the SAM. These results suggest that the rice KN1-type class 1 homeobox genes function cooperatively to establish the SAM before shoot formation and that after shoot formation, their functions differ.

水稻KN1类型的同源异型盒基因在胚、芽、花发育时的区域表达

本研究对水稻KNOTTED1(KN1)类型的第一类同源异型盒基因家族的基因成员进行了分离,并对基因的序列和表达模式进行分析。通过系统发生分析和将基因定位到水稻基因组上的工作表明,所有分离到的同源异型盒基因都有1个或2个玉米的直接同源物。所有作者进行检测的同源异型盒基因都在胚中严格限制的区域内表达,在可见的器官形成之前决定什么部位最终能够发育成顶端生长点(SAM)。一些基因在这些区域的时空表达存在着显著的差异。芽形成以后,在SAM区域能检测到这些同源异型盒基因的表达模式。这些结果表明水稻KN1类型的第一类同源异型盒基因在芽形成之前对SAM的建立发挥着协同作用,在芽形成后发挥着不同的功能。


基因列表
  矮秆基因;水稻同源异型盒基因 d6; OSH15;Oskn3
  水稻同源异型盒基因 OSH6
  同源异型盒基因 OSH1; Oskn1
  同源异型盒基因 OSH71; Oskn2
  水稻同源异型盒基因 OSH43
  水稻同源异型盒基因 OSH3
  水稻同源异型盒基因 OSH10