Identification and characterization of Bph14, a gene conferring resistance to brown planthopper in rice

Bo Du, Weilin Zhang, Bingfang Liu, Jing Hu, Zhe Wei, Zhenying Shi, Ruifeng He, Lili Zhu, Rongzhi Chen, Bin Han, Guangcun He
Proceedings of the National Academy of Sciences, 2009, 106(52): 22163-22168  DOI: 10.1073/pnas.0912139106     追溯原文......本站官方QQ群:62473826
herbivore; insect-resistance gene; CC-NB-LRR protein; antibiosis; salicyclic acid signaling

Planthoppers are highly destructive pests in crop production worldwide. Brown planthopper (BPH) causes the most serious damage of the rice crop globally among all rice pests. Growing resistant varieties is the most effective and environment-friendly strategy for protecting the crop from BPH. More than 19 BPH-resistance genes have been reported and used to various extents in rice breeding and production. In this study, we cloned Bph14, a gene conferring resistance to BPH at seedling and maturity stages of the rice plant, using a map-base cloning approach. We show that Bph14 encodes a coiled-coil, nucleotide-binding, and leucine-rich repeat (CC-NB-LRR) protein. Sequence comparison indicates that Bph14 carries a unique LRR domain that might function in recognition of the BPH insect invasion and activating the defense response. Bph14 is predominantly expressed in vascular bundles, the site of BPH feeding. Expression of Bph14 activates the salicylic acid signaling pathway and induces callose deposition in phloem cells and trypsin inhibitor production after planthopper infestation, thus reducing the feeding, growth rate, and longevity of the BPH insects. Our work provides insights into the molecular mechanisms of rice defense against insects and facilitates the development of resistant varieties to control this devastating insect.



  褐飞虱抗性基因 Bph14; Qbp1