Identification and characterization of Bph14, a gene conferring resistance to brown planthopper in rice

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Bo Du, Weilin Zhang, Bingfang Liu, Jing Hu, Zhe Wei, Zhenying Shi, Ruifeng He, Lili Zhu, Rongzhi Chen, Bin Han, Guangcun He
Proceedings of the National Academy of Sciences, 2009, 106(52): 22163-22168  DOI: 10.1073/pnas.0912139106     追溯原文......本站官方QQ群:62473826
herbivore; insect-resistance gene; CC-NB-LRR protein; antibiosis; salicyclic acid signaling

Planthoppers are highly destructive pests in crop production worldwide. Brown planthopper (BPH) causes the most serious damage of the rice crop globally among all rice pests. Growing resistant varieties is the most effective and environment-friendly strategy for protecting the crop from BPH. More than 19 BPH-resistance genes have been reported and used to various extents in rice breeding and production. In this study, we cloned Bph14, a gene conferring resistance to BPH at seedling and maturity stages of the rice plant, using a map-base cloning approach. We show that Bph14 encodes a coiled-coil, nucleotide-binding, and leucine-rich repeat (CC-NB-LRR) protein. Sequence comparison indicates that Bph14 carries a unique LRR domain that might function in recognition of the BPH insect invasion and activating the defense response. Bph14 is predominantly expressed in vascular bundles, the site of BPH feeding. Expression of Bph14 activates the salicylic acid signaling pathway and induces callose deposition in phloem cells and trypsin inhibitor production after planthopper infestation, thus reducing the feeding, growth rate, and longevity of the BPH insects. Our work provides insights into the molecular mechanisms of rice defense against insects and facilitates the development of resistant varieties to control this devastating insect.

水稻抗褐飞虱基因Bph14的分离和鉴定

稻飞虱是世界范围内对农作物生产具有很高破坏性的害虫。在所有的水稻害虫中,褐飞虱是对水稻造成伤害最严重的的害虫。种植抗褐飞虱的水稻品种是对付褐飞虱最有效同时也是对环境不造成影响的一种策略。至今已有19个抗褐飞虱的基因报道并应用与不同地区的水稻育种和生产中。本文,我们通路图位克隆的方法克隆了一个在水稻苗期和成熟期都抗褐飞虱的基因Bph14Bph14编码一个含有卷曲螺旋、核苷酸结合以及富含亮氨酸重复序列的蛋白。序列比对表明Bph14具有一个独特的富含亮氨酸重复序列,可能起到识别褐飞虱入侵和激活抗性反应的功能。Bph14主要在褐飞虱摄食部位-维管束表达。Bph14的表达在褐飞虱侵染之后激活了水杨酸信号传导通路,诱导韧皮部细胞的胼胝质沉积以及胰蛋白酶抑制剂的产生,因此降低了褐飞虱的取食、生长速率和寿命。我们的研究结果为了解水稻抗虫的分子机理提供了新的见解,同时有助于培育抵抗褐飞虱这种破坏性极强的害虫的水稻品种。


基因列表
  褐飞虱抗性基因 Bph14; Qbp1