The Effect of the Crosstalk between Photoperiod and Temperature on the Heading-Date in Rice

4.351
Weijiang Luan, Huizhe Chen, Yaping Fu, Huamin Si, Wen Peng, Susheng Song, Wenzhen Liu, Guocheng Hu, Zongxiu Sun, Daoxin Xie, Chuanqing Sun
PLoS ONE, 2009, 4(6): e5891  DOI: 10.1371/journal.pone.0005891;      追溯原文......本站官方QQ群:62473826

Photoperiod and temperature are two important environmental factors that influence the heading-date of rice. Although the influence of the photoperiod on heading has been extensively reported in rice, the molecular mechanism for the temperature control of heading remains unknown. This study reports an early heading mutant derived from tissue culture lines of rice and investigates the heading-date of wild type and mutant in different photoperiod and temperature treatments. The linkage analysis showed that the mutant phenotype cosegregated with the Hd1 locus. Sequencing analysis found that the mutant contained two insertions and several single-base substitutions that caused a dramatic reduction in Hd1mRNA levels compared with wild type. The expression patterns of Hd1 and Hd3a were also analyzed in different photoperiod and temperature conditions, revealing that Hd1 mRNA levels displayed similar expression patterns for different photoperiod and temperature treatments, with high expression levels at night and reduced levels in the daytime. In addition, Hd1 displayed a slightly higher expression level under long-day and low temperature conditions. Hd3a mRNA was present at a very low level under low temperature conditions regardless of the day-length. This result suggests that suppression of Hd3a expression is a principle cause of late heading under low temperature and long-day conditions.

光周期和温度的互作会影响水稻的抽穗期

光周期和温度是影响水稻抽穗期的两个重要环境因素。尽管光周期对水稻抽穗的影响已经被广泛报道,但是温度控制抽穗的分子机理仍不清楚。本文报道了一个来自水稻组织培养产生的早花突变体,并分析了野生型植株和突变体在不同光周期和温度处理下的抽穗期。连锁分析表明突变体表型与Hd1位点共分离。序列分析发现,与野生型相比,突变体包含两个片段插入和多个单碱基替换,进而引起Hd1的mRNA水平显著降低。在不同光周期和温度条件下对Hd1Hd3a的表达模式分析表明,不同光周期和温度处理时,Hd1的mRNA水平表现出相似的表达模式,夜晚具有高表达,而白天表达水平降低。此外,在长日照和低温条件下,Hd1表现出较高的表达水平。在低温条件下,无论日照长短,Hd3a均表现出较低的mRNA水平。这些结果表明,抑制Hd3a的表达是引起低温和长日照条件下晚抽穗的主要原因。


基因列表
  抽穗期基因 Hd1
  成花素基因;抽穗期基因;生育期基因;光周期敏感基因;感光基因 Hd3a; FT