Seed size is determined by the combinations of the genes controlling different seed characteristics in rice

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Song Yan, Guihua Zou, Sujuan Li, Hua Wang, Heqin Liu, Guowei Zhai, Peng Guo, Hongmiao Song, Changjie Yan, Yuezhi Tao
Theoretical and Applied Genetics, 2011, 123(7): 1173-1181  DOI: 10.1007/s00122-011-1657-x     追溯原文......本站官方QQ群:62473826

Rice seed size is an important agronomic trait in determining the yield potential, and four seed size related genes (GS3, GW2, qSW5/GW5 and GIF1) have been cloned in rice so far. However, the relationship among these four genes is still unclear, which will impede the process of gene pyramiding breeding program to some extent. To shade light on the relationship of above four genes, gene expression analysis was performed with GS3-RNAi, GW2-RNAi lines and CSSL of qSW5 at the transcriptional level. The results clearly showed that qSW5 and GW2 positively regulate the expression of GS3. Meanwhile, qSW5 can be down-regulated by repression of GW2 transcription. Additionally, GIF1 expression was found to be positively regulated by qSW5 but negatively by GW2 and GS3. Moreover, the allelic effects of qSW5 and GS3 were detailedly characterized based on a natural population consisting of 180 rice cultivars. It was indicated that mutual interactions exist between the two genes, in which, qSW5 affecting seed length is masked by GS3 alleles, and GS3 affecting seed width is masked by qSW5 alleles. These findings provide more insights into the molecular mechanisms underlying seed size development in rice and are likely to be useful for improving rice grain yield.

控制水稻不同籽粒性状的基因组合控制水稻籽粒大小

水稻籽粒大小是控制产量潜能的重要农艺性状,到目前为止,水稻中已有4个控制籽粒大小相关的基因(GS3GW2qSW5/GW5GIF1)被克隆。然而,这4个基因的相互关系依然未知,这在一定程度上阻碍了对这些基因在育种中的聚合应用。为了研究这4个基因的关系,对GS3-RNAi、GW2-RNAi系和qSW5的CSSL进行了基因表达分析。结果很明显:qSW5GW2正调控GS3GW2的表达能够下调qSW5的表达,qSW5正调控GIF1的表达,而GW2GS3负调控GIF1的表达。此外,基于一个含有180个水稻品种的自然群体,详细分析了qSW5GS3的等位基因作用,结果表明这2个基因之间存在相互作用,qSW5对粒长的作用受到GS3的影响,GS3对粒宽的作用受到qSW5的影响。本研究为更加深入理解水稻籽粒大小发育的分子机制及提高水稻产量提供了有用的信息。


基因列表
  粒长粒重主效控制基因 GS3
  籽粒体型控制基因;细胞壁转化酶 GIF1; OsCIN2
  粒宽和粒重主效控制基因 gw5
  粒宽和粒重主效控制基因 GW2
  粒宽基因 qSW5