Using bulked extremes and recessive class to map genes for photoperiod-sensitive genic male sterility in rice

Qifa Zhang, B. Z. Shen, X. K. Dai, M. H. Mei, M. A. Saghai Maroofo, Z. B. Li
Proceedings of the National Academy of Sciences, 1994, 91(18): 8675-8679     追溯原文......本站官方QQ群:62473826

Photoperiod-sensitive genic male sterile (PS-GMS) rice has a number of desirable characteristics for hybrid rice production. In this study we made use of a published rice genetic linkage map to determine the locations of PSGMS genes and we have characterized the effects of these genes on sterility by using molecular markers. A two-step approach was designed for mapping the genes: (i) identifying possible PSGMS gene-containing chromosome regions with bulked DNA from extreme fertile and extreme sterile plants of a very large F2 population and (ii) determining the map locations of the genes in extreme sterile individuals. We show that this mapping method is much more cost effective and statistically efficient than using a random sample of an F2 population. We identified two chromosomal regions each containing a PSGMS locus, one designated pms1 on chromosome 7 and one designated pms2 on chromosome 3. The existence of these two loci was confirmed by a large sample assay and with data on rationing progenies of the F2 plants. A marker-based analysis shows that the effect of pms1 is 2-3 times larger than that of pms2 and that dominance is almost complete at both loci. Implications in the breeding of PSGMS rice lines are discussed.


基因列表
  光敏雄性核不育基因 pms1
  光敏雄性核不育基因 pms2