·常规信息
基因(座)名称钙依赖性蛋白激酶
Calcium-dependent protein kinase
基因符号OsCDPK1; OsCPK11; OsCDPK13; OsCPK7
所在染色体3 (已克隆)

OsCDPK1(AY158077, Ho et al. 2013), OsCDPK13(AB078634, Yang et al. 2003), OsCPK11(X81393, Breviario et al. 1995), OsCPK7(AK066500, Asano et al. 2005), 位于同一基因位点...

【突变体表型】

OsCDPK1功能获得转基因水稻株高和籽粒大小均降低,粒长和粒宽下降7%和5%,千粒重降低24%,但耐旱性增加,而OsCDPK1-RNAi水稻株高和籽粒大小增加,粒宽和粒重增加13%和8%,千粒重增加10%,耐旱性降低(Ho et al. 2013)。

【定位与克隆】

 

【时空表达谱】

OsCDPK1在早期幼苗生长阶段有较强表达,并特异受蔗糖饥饿诱导,不受ABA、NaCl和甘露醇的诱导(Ho et al. 2013)。

OsCDPK13在根和叶鞘等组织有较高表达,受冷和赤霉素处理诱导(Yang et al. 2003; Abbasi et al. 2004)。

【亚细胞定位】

 

【生物学功能】

种子萌发后,OsCDPK1转导来源于糖饥饿和GA的Ca2+信号,负调控赤霉素合成,通过激活14-3-3蛋白GF14c表达,抑制干旱损伤,参与水稻幼苗发育的所有重要功能(Ho et al. 2013)。

【相关登录号】
contigs及其产物:AP000615BAA85396, AP014959BAS82107
cDNAs及其产物:AY158077AAN76358, X81393CAA57156, AF048691AAC05270, AB078634BAC19839, AK066500
参考基因组位点:Os03g0128700(RAP-DB, Gramene←→ LOC_Os03g03660(本地MSU-RGAP←→ LOC4331490(NCBI)
参考基因组产物:XM_015776049XP_015631535, XM_015776050XP_015631536
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·ONTOLOGY及相关基因
表型特征株高(TO:0000207), 籽粒大小(TO:0000397), 耐寒性(TO:0000303), 粒宽(TO:0000402), 粒长(TO:0000734), 耐旱性(TO:0000276), 赤霉素含量(TO:0002675), 千粒重(TO:0000592)
分子功能钙离子结合(GO:0005509), 钙依赖性蛋白激酶活性(GO:0010857)
生物进程赤霉素应答(GO:0009739), 低温胁迫应答(GO:0009409), 钙信号传导(GO:0019722), 赤霉素生物合成负调控(GO:0010373), 幼苗发育(GO:0090351), 碳饥饿应答(GO:0090549)
·参考文献
1Shin-Lon Ho;Li-Fen Huang;Chung-An Lu;Siou-Luan He;Chun-Chin Wang;Sheng-Ping Yu;Jychian Chen;Su-May Yu
  Sugar starvation- and GA-inducible calcium-dependent protein kinase 1 feedback regulates GA biosynthesis and activates a 14-3-3 protein to confer drought tolerance in rice seedlings
  Plant Molecular Biology, 2013, 81(4-5): 347-361
2Takayuki Asano;Naoki Tanaka;Guangxiao Yang;Nagao Hayashi;Setsuko Komatsu
  Genome-wide Identification of the Rice Calcium-dependent Protein Kinase and its Closely Related Kinase Gene Families: Comprehensive Analysis of the CDPKs Gene Family in Rice
  Plant and Cell Physiology, 2005, 46(2): 356-366
3Fida Abbasi;Haruko Onodera;Seiichi Toki;Hiroshi Tanaka;Setsuko Komatsu
  OsCDPK13, a calcium-dependent protein kinase gene from rice, is induced by cold and gibberellin in rice leaf sheath
  Plant Molecular Biology, 2004, 55(4): 541-552
4Guangxiao Yang;Shihua Shen;Shihai Yang;Setsuko Komatsu
  OsCDPK13, a calcium-dependent protein kinase gene from rice, is induced in response to cold and gibberellin
  Plant Physiology and Biochemistry, 2003, 41(4): 369-374
5Milo Frattini;Laura Morello;Diego Breviario
  Rice calcium-dependent protein kinase isoforms OsCDPK2 and OsCDPK11 show different responses to light and different expression patterns during seed development
  Plant Molecular Biology, 1999, 41(6): 753-764
6Diego Breviario;Laura Morello;Silvia Gianì
  Molecular cloning of two novel rice cDNA sequences encoding putative calcium-dependent protein kinases
  Plant Molecular Biology, 1995, 27(5): 953-967
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